To study the ultrasonic extraction technology of rutin from Flos Sophorae with ethanol solution and β-cyclodextrin ultrasonic-assisted extraction process.Methods:HPLC method was established for the determination of rutin by orthogonal test design with the two extraction methods to calculate the extraction rate and optimize the extraction process of rutin from Flos Sophorae.Results:The optimal conditions for ultrasonic extraction with ethanol solution were determined as extraction for 30 minutes with 70%-concentrated and 50ml ethanol. The highest extraction rate was 19.25%. The optimal conditions for β-cyclodextrin ultrasonic-assisted extraction were as follows: the extraction time is set at 30 min, the weight of β-cyclodextrin is 3.0g, water volume is 50ml and the highest extraction rate was 18.32%. Conclusion: This method is simple and efficient, extraction rate is high, it’s worth using widely.