To select an effective barcode for identifying Herba Aconiti Bonga and its adulterants using DNA barcoding technology.Methods:This study compared ITS and ITS2 sequences and analyzed genetic distances，the variations of inter-and intra-species.Neighbor-joining tree was also constructed for evaluate their identification efficiencies based on the sequences from experimentaland GenBank database.Results:The results showed that ITS and ITS2 sequences had the same amplication efficiency.ITS sequence had a better performance than ITS2 on species identification efficiency using blast and nearest distance methods.The two barcodes showed no significant difference on the identification uing NJ tree to distinguish Herba Aconiti Bonga and its adultrants.However results demonstrated that ITS can also identify all species among adultrants of Herba Aconiti Bonga.Conclusion:ITS sequence can be used as the effective barcode to identifyHerba Aconiti Bonga and its adulterants.