Psoraleae Fructus has toxic effects on liver and kidney and its adulterants including Datura stramonium，Hyoscyamus niger，D.metel，Crotalaria pallid are also tested to have toxic ingredients.Psoralea corylifolia shares similar morphological characters with its adulterants，which can easily lead to misuses and greatly threaten the human health.This study aimed to identify P.corylifolia and its adulterants rapidly and accurately using DNA barcoding method based on ITS2 sequence.Methods:Genomic DNA of P.corylifolia and its adulterants were extracted and then the ITS2 sequences were obtained by direct PCR amplification and sequenced bi-directionally.The intra-and inter-specific genetic distances were analyzed using software MEGA 6.0，and the phylogenetic tree was constructed by using the neighbor-joining(NJ)method.Results:The ITS2 sequence length of P.corylifolia was 233bp，and the G+C content was 69.5%.The intra-specific variations were highly conservative.There were many variation sites of ITS2 sequences between P.corylifolia and its adulterants.The results showed that the minimum inter-specific divergence was 0.5321，which was larger than the maximum intra-specific divergence.The NJ tree indicated that P.corylifolia could be distinguished from its adulterants obviously.Conclusion:The ITS2 region can be used as a barcode for identification of P.corylifolia，which provide a new technique for the authentication of germplasm resources and clinic safety of medicinal use.