To observe the effect of Zhi Dan Hua Yu prescriptionon CGRP、ET-1 and TNF-αof lung injury rats induced byendotoxin.Methods:A total of 84 SD rats withhalf males and half females were divided into control group,model group,Zhi Dan Hua Yu prescription group(33 g/kg,16.5 g/kg,8.25 g/kg)and dexamethasone group by random,detecting the changes ofwatercontent inlung injury rats’ lung；utilizing radioimmunoassay to detecting changes of ET and CGRP in rat plasma.Results:Compared to control group，lung water content in model group rats were significantly increased(P<0.01),Zhi Dan Hua Yu prescription(33 g/kg,16.5 g/kg)and dexamethasone obviously reduced water content in lunginjury rats.The endothelin(ET)and calcitonin gene peptide(CGRP)in rat plasmaof model group were significantly increased(P<0.01)compared to that of control group;dexamethasone reduced ET content(P<0.01)and Zhi Dan Hua Yu prescription(16.5 g/kg,8.25 g/kg)reduced ET contant apparently(P<0.01); CGRP contant in rat plasma of Zhi Dan Hua Yuprescription(33 g/kg)were increased significantly(P<0.01); The content of tumor necrosis factor-α(TNF-α)was increased apparently(P<0.01)in comparison with that of control group; dexamethasone and Zhi Dan Hua Yu prescription(33 g/kg,16.5 g/kg,8.25 g/kg)reduced TNF-αin rat plasma obviously(P<0.01).Conclusion:Zhi Dan Hua Yu prescriptionprotect endotoxin-induced lung injury rats by increase CGRP content and reduce ET-1 and TNF-αcontent in rat plasma.