To observe the effect of Zhi Dan Hua Yu formula on acute-injured lung of rats induced by LPS.Methods:A total of 72 SD rats withhalf males and half females were divided into control group,model group,Zhi Dan Hua Yu group(33 g/kg,16.5 g/kg,8.25 g/kg)and dexamethasone group by random.Acute lung injury induced by instillation of LPS; HE staining; to observe the pathological changes of lung tissue and give a pathological score of lung injury by light microscope; to detect the content of lung tissue inflammation factor interleukin-1 beta(IL-1)and interleukin 6(IL-6)，tumor necrosis factor alpha(TNF alpha).Results:Compared with normal group，the Model group rats lung tissue pathological score were increased，TNF-a，IL-1 and IL-6 were significantly increased(P<0.01).compared with the model group rats zhidanhuayu prescription 33 g/kg and dexamethasone group rat lung tissue pathological score were significantly decreased(P<0.05).dexamethasone and zhidanhuayu prescription(33，16.5，8.25 g/kg)can significantly reduce the lung tissue TNF alpha，IL-6.zhidanhuayu prescription 16.5 g/kg also can significantly reduce the content of IL-1，which is more obvious than that of model group(P<0.01).Conclusion:Zhidanhuayu formula could reduce the acute lung injury induced by LPS，the effect mechanism may be related to the decrease of TNF-a，IL-6 and IL-1 content.