三七酚类成分特征图谱质量表征关联评价研究
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十二五国家科技支撑计划项目(编号:2012BAI29B06);北京中医药大学创新团队资助项目(编号:2011-CXTD-12)


Quality Representation of Characteristic Chromatogram and Correlation Analysis of Phenolic Components in Panax notoginseng
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    摘要:

    采用HPLC-PDA法建立三七酚类成分特征图谱,筛选9个特征峰并通过相对保留时间表征其质量关联性,同时测定对羟基苯甲酸、槲皮素、山柰酚、异鼠李素含量,以对羟基苯甲酸为对照表征酚酸类含量,采用分光光度法测定三七中总酚含量,从而对三七酚类成分含量及其相对比值进行质量表征,然后对三七中各酚类成分含量进行关联分析,从而发现各成分含量的关联性、质量分布规律性,同时将不同批次的药材与经过药效验证的参比药材进行关联度分析,结合关联度分析,筛选出质量优佳三七药材;运用本方法可全面精确的控制和评价三七的质量。

    Abstract:

    Characteristic chromatogram of Phenolic Components in Panax notoginseng was established by HPLC-PDA method and 9 featured peaks were screened. Their quality correlation was represented by relative retention schedule. Contents of P-hydroxybenzoic acid, quercetin, kaempferol, isorhamnetin and total phenols were determined, taking p-hydroxybenzoic acid as the control content agent. The total phenolic content of Panax notoginseng was determined by spectrophotometry. Quality representation was made with the contents of phenols in Panax notoginseng and their relative ratio. Correlation analysis of content of each phenol in Panax notoginseng was performed to summarize correlation and quality distribution rules among components in Panax notoginseng. The correlation between different batches of medicinal materials and referential materials which had pharmacodynamics validation was analyzed. The optimal Panax notoginseng could be selected based on the results of correlation analysis. This method can fully and accurately control and evaluate the quality of Panax notoginseng.

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杨元,陈唯,张芳,孔静,孙道涵,冯朵,杨书娟,申立峰,胡少伟,周德勇,潘婷,姜艳艳,石任兵.三七酚类成分特征图谱质量表征关联评价研究[J].世界中医药,2016,(05).

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  • 收稿日期:2016-01-14
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  • 在线发布日期: 2016-05-18
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