To identify Centella asiatica and adulterated Species by using GenBank nucleicacid database matK sequences.Methods:Based on GenBank Nucleotide Database，matK sequences of Centella asiatica and adulterated Species were downloaded.The SNP sites were checked by ClustalX 2.1 software，K2P Distances were calculated using MEGA 5.0 software and neighbor-ioining（NJ）phylogenetic tree was established.Results:There many specific SNP sites can be used to molecular authentication of Centella asiatica and adulterated Species.K2P genetic distance between Centella asiatica and adulterated Species is between 0.242～0.293，which was far more than intraspecific genetic distance of Centella asiatica（0.001）and adulterated Species（0.004）.Results of NJ phylogenetic tree showed that Centella asiatica can be separated from adulterated Species and classified into a separate one.Conclusion:matK sequence is suitable for the identification of Centella asiatica and adulterated Species and provides reference for quality and safety identification of Centella asiatica and adulterated Species.