To observe the effect of pricking blood on colleterals therapy on AFP and SF in rats model of primary liver cancer (HCC),and explore its preventive effect and related mechanism.Methods:Forty healthy Wistar rats were randomly divided into control group (n=10),model group (n=15) and pricking blood group (n=15).In addition to the control group,the other two groups were injected with DEN (Diethylnitrosamine) intraperitoneally to establish liver cancer model.Rats in pricking blood group was performed of blood letting on the superficial veins of hind leg along the liver meridian,0.5 mL each time and 2 times per week,till the eighteenth week.At the end of the week,taking 5 mL blood from the abdominal aorta and using ELESA method to determine ALT (Alamine Amino Transferase),ALP (Alkaline Phosphatase),GGT (Gamma Glutamyl Transpeptidase),AFP and SF levels in serum.Results:Compared with the control group,the serum levels of ALT,ALP,GGT,AFP and SF in model group were significantly increased,and the difference was statistically significant (P<0.05).Compared with the model group,the serum levels of ALT,ALP,GGT,AFP and SF in the prevention group were lower,and the difference was statistically significant (P<0.05).Conclusion:Blood pricking on colleterals therapy has inhibitory effect on HCC,which might due to the fact that it can decrease the liver serum levels and tumor signals AFP and SF.