To study the clinical effects of Chinese medicine enema for the treatment of Ⅲ type prostatitis. Methods: Eighty cases of Ⅲ type prostatitis were randomly divided into two groups, with 40 cases in each group. Patients were respectively given conventional treatment of western medicine (the control group) and conventional western medicine treatment combined with Chinese medicine enema therapy (the observation group). Clinical symptoms integral, prostate fluid white blood cells, the concentration of lecithin corpuscle in serum estradiol (E2), and testosterone (T) conditions of the two groups before and after treatment were evaluated. Results: clinical symptoms integral of the two groups were significantly improved after treatment (P<0.05), and that of the observation group improved more significantly (t=4.2425, P<0.05). Prostatic fluid leukocyte 17 beta, lecithin corpuscle content and serum E2, T of the two groups were significantly improved (P<0.05) and that of the observation group improved more significantly (P<0.05). Conclusion: Chinese medicine enema treatment may effectively improve the clinical effect of treatment for Ⅲ type prostatitis and regulate sex hormone of patients with Ⅲ type prostatitis.