To observe clinical curative effect of Danzhi Xiaoyao Powder in the therapy of ganyu qizhi type of neovascularglaucoma（NVG） and deeply investigate the relationship between this effect and HIF-1/VEGF/Notch1 pathway. Methods: A total of 42 patients NVG were randomly divided into into 2 groups，21 patients in treatment group were treated with modified Danzhi Xiaoyao Powder and iop drugs，21 patients in control group were treated with only iop drugs.Observe the two groups after treatment of 4 weeks, intraocular pressure, visual ，view and new blood vessels.The expression of HIF-1a、VEGF、Notch1 at the protein level was assessed by Western Blot．Results: Significant differences were observed in terms of intraocular pressure, visual, view and new blood vessels (P<0.05). Compared with the control group, the expression of HIF-1a, VEGF, Notch1 level in the treatment group were significantly decreased (P<0.05). Conclusion: Danzhi Xiaoyao Powder in the treatment of NVG may effectively control the intraocular pressure, improve eyesight and restrain new blood vessels. It is a safe and effective treatment, worthy of clinical popularization and application. Its mechanism may be related to the regulation of HIF-1/VEGF/Notch1 signaling pathway.