To observe the effects of Supplemented Liang-fu Pill(SLFP) combined with 5-FU on TGF-β1、IL-17 level in serum of SGC-7901 tumor bearing nude mice. Methods: The tumor-bearing nude mice models with human gastric cancer cell line SGC-7901were established and randomly divided into six groups: model group, 5-FU group, combination group and SLFP in high, medium, low dose groups. The dosage of SLFP in high, medium, low dose groups were 10 g·d-1·kg-1、5 g·d-1·kg-1、2.5 g·d-1·kg-1 respectively. The 5-FU group′s dosage is 17 mg·d-1·kg-1, the model group were given equal normal saline and the combination group were given Supplemented Liang-Fu Pill (5 g·d-1·kg-1) and 5-FU(17 mg·d-1·kg-1). The mice were treated for 10 days and then detected for the level of TGF-β1 and IL-17 in serum of SGC-7901 tumor bearing nude mice with the method of ELISA. Results: The levels of TGF-β1 and IL-17 in 5-FU group, combination group, SLFP in high, medium, low dose groups were significantly lower than those in model group (P<0.01). The levels of serum TGF-β1 in the combination group was not significantly different compared with that of SLFP in high dose group and the 5-FU group (P<0.05). But there was significant different compared with SLFP in high, medium and dose groups. There were no significant difference of the levels of serum IL-17 in each group(P>0.05). Conclusion: Inflammatory cytokine TGF-β1, IL-17 may result in gastric cancer. The SLFP combined with 5-FU maybe inhibit the expression of TGF-β1and IL-17 in the serum of human gastric cancer cell SGC-7901 tumor bearing nude mice, which may affect the growth of gastric cancer by regulating the expression of inflammatory cytokines.