To observe the effects of Salvia miltiorrhiza on apoptosis of human gastric cancer SGC-7901 cells. Methods: To test the effects of different concentrations of Salvia miltiorrhiza, different concentrations of 5-FU, and the mixtures on apoptosis rate of human gastric cancer SGC-7901 cells by flowing cytometry after AnnexinV-FITC/PI staining. To observe the effects of different concentrations of Salvia miltiorrhiza, different concentrations of 5-FU, and the mixtures on number of apoptosis of human gastric cancer SGC-7901 cells by fluorescence microscope. Results: 1) The apoptosis rate of human gastric cancer SGC-7901 cells that disposed with different concentrations of Salvia miltiorrhiza is higher than the control group, and the apoptosis rate has raised with the increase of concentration of Salvia miltiorrhiza.2) The apoptosis rate of human gastric cancer SGC-7901 cells that disposed with combination therapy group is higher than that of the group disposed by only Salvia miltiorrhiza or 5-FU, and the apoptosis rate increased with the increase of concentration of Salvia miltiorrhiza. 3)Using the Fluorescence microscopy, we can find that the number of the intact cells in Salvia miltiorrhiza combined with 5-Fu groups were all lower than the control group, and the number of apoptotic cells has increased with the drug concentration increasing. Conclusion: Salvia miltiorrhiza can cause human gastric cancer SGC-7901 cell morphological changes and accelerate 5-FU induce the apoptosis of cell, which was correlated with dose.