Observe the difference between pulse-taking depths (Fu, Zhong, and Chen) and pulse-taking positions (Cun, Guan, and Chi).Methods:A PDS-1 Shiatsu-pulse-diagnosis instrument was used to collect the pulse waveform diagrams of 51 students′ left-right hands between pulse-taking depths by using three fingers on cun, guan and chi. The parameters of pulse waveform diagrams are extracted by time domain analysis method. The data were analyzed by the method of multi factor analysis of variance.Results:Different hands, pulse-taking depths and pulse-taking positions have an effect on pulse waveform diagrams. When h1~h5 is used as the observation index, there is an interaction between hands and pulse-taking depths, while there is an interaction between pulse-taking depths and pulse-taking positions.Conclusion:The differences in pulse waveform diagrams exist between three positions and nine indicators. PDS-1 Shiatsu-pulse-diagnosis instrument could be used to obtain pulse waveform diagrams.