Pseudomonas aeruginosa is an opportunistic pathogenic bacterium that causes acute or chronic infection, one of its drug resistance factor is to form biological film easily. According to recent studies, Chinese material medica has significant inhibitory effect on pseudomonas aeruginosa biological film formation. The author reviewed the biological film formation cycle of pseudomonas aeruginosa, the major regulatory signal system for biofilm formation and the impact of Chinese material medica on it.