To investigate the effects of Fuzheng Xiaoai Decoction on cell proliferation, invasion and apoptosis in tissues of breast cancer, and its relevant apoptotic molecular mechanisms. Methods:A total of 66 cases of breast cancer were randomly divided into the control group (30 cases) and the treatment group (36 cases). The control group received chemotherapy drugs doxorubicin and cyclophosphamide. On the basis of the control group, the treatment group were treated with Fuzheng Xiaoai Decoction once a day for nine weeks. Ki67, MMP2 and Fas protein expression levels were determined by immunohistochemical technology. The protein levels of Bax, Bcl-2 were detected by western blotting. Results:The Fas expressions of breast cancer tissues were significantly higher in treatment group than the control group (P<0.05). The Ki67, MMP2 expressions in breast cancer tissues were lower in treatment group compared with the control group (P<0.05). Results of western blotting indicated that the protein level of Bax in breast cancer tissues of the treatment group increased significantly compared with the control group, but the Bcl-2 expression level decreased significantly, the differences were statistically significant (both P<0.05). Conclusion:Fuzheng Xiaoai Decoction can inhibit the proliferation and invasion breast cancer cells, promote the apoptosis of breast cancer cells, which may be the molecular mechanism of Fuzheng Xiaoai Decoction in the treatment of breast cancer.