To observe the effect of the Kanglaite Injection in treatment of non-small cell lung cancer, and to discuss the regulation of T lymphocyte subsets. Methods:A total of 90 cases of non-small cell lung cancer in our department from August 2015-January 2016, were randomly divided into control group and observation group, 45 cases in each group. The control group received glucose injection of 250 mL twice a day. Observation group were given the Kanglaite Injection twice a day. After 15 days of treatment, the clinical efficacy, all immune factors score, adverse reactions, clinical benefit analysis and serum IL-2, IL-6, IL-10 and TNF-α level changes were compared. Results:1)After treatment, the effective rate of observation group was 86.67%, the control group 68.89%, the difference was significant (P<0.05）；2) Before treatment, there was no significant difference on immune function between the two groups(P>0.05), after 15 days′ treatment, the immunized kinetic energy of observation group slightly improved, but control group had no changes, the difference was statistically significant (P<0.05）；3) During the treatment, patients in both two groups experienced different adverse reactions, such as abdominal pain, diarrhea, nausea, vomiting, white blood cells, platelets, hemoglobin and reduction, there was one case (2.22%)in the control group and two cases (4.44%) in observation group, the difference was not statistically significant, showing that the medicine did not increase the side effects (P>0.05). There were significantly more cases that experienced KPS and body weight changes in the observation group than control group (P<0.05）； 4) Before treatment, there were no differences on plasma IL-2, IL-6, IL-10 and TNF-α level between two groups(P>0.05). But after 15 days′ treatment, the index decreased, and the observation group decreased more significantly. Conclusion:Kanglaite Injection can significantly improve the immunity of non-small cell lung cancer patients with surgery, radiotherapy and chemotherapy, with significant effect and less adverse reactions, which is worth promoting.