To establish the quality standard of Zhong-feng-ning mixture.Methods:TLC was used for the qualitative identification of paeoniae radix rubar, acori tatarinowii rhizome and polygoni multiflori radix. The samples were separated on the Agilent ZORBAX SB C18 column. The mobile phase was set as methanol-0.05% monopotassium phosphate（28：72）and the detection wave length was set at 230 nm.Results:The TLC spots of paeoniae radix rubar, acori tatarinowii rhizoma, polygoni multiflori radix were clear and well-separated without interference of negative samples. The linear relationship of peaoniflorin was good in the range of 0.1852-0.9260 μg (r=0.999 9). The average recovery rate was 98.55%. (RSD=1.16).Conclusion:The method is simple, accurate and fast with great repeatability, high specificity and good repeatability, which can be used for the quality control of Zhong-feng-ning mixture.