To study and optimize the extraction process of seed oil of Hippophae rhamnoides L.by microwave extraction technology. Methods: The orthogonal design method was used to optimize the extraction process seed oil of Hippophae rhamnoides L. by microwave extraction technology with the oil yield as the index and microwave power, microwave time and liquid to solid ratio were taken as study factors. Results: The optimum extraction process was as follows: microwave power at 539 W, microwave time at 7 min and liquid to solid ratio at 8. The extraction rate of oil was 89.36% under this extraction conditions. Conclusion: Microwave extraction technology may have good extraction yield and high extraction efficiency compared with traditional extraction methods. It is suitable to apply microwave technology into the extraction process of seed oil of Hippophae rhamnoides L. with the purpose of increasing the yield and reducing the extraction time.