To explore the way to establish the kidney and blood stasis model in tree shrew. Methods: Tree shrews were randomly divided into 3 groups: normal group, model group 1 (injection of Hydrocortisone and Adrenaline) and model group 2 (injection of Hydrocortisone and Carrageenan). The kidney deficiency and blood stasis model was evaluated by observation of representation of tree shrews, detecting visceral index, hemorheology parameters and the level of hypothalamus-pituitary-target gland axis (thyroid axis, gonad axis). Results: The animal been treated all appeared “exhausted” phenomenon, the kidney, prostate, spleen, testis of the model groups were swelling, but the thyroid in model group 2 was atrophied. The levels of E2 and T in model group 1, levels of FT3、FT4 and E2 in model group 2 declined remarkably. The results of hemorheology showed that the blood viscosity and hematocrit increased in model group 1. Conclusion: The tree shrews model of kidney deficiency and blood stagnation can be established by injection of hydrocortisone and adrenaline.