To explore the optimum dose in tree shrews model of blood stasis syndrome induced by carrageen glue via observation of representation and hemorheology parameters of tree shrews. Methods: A total of 32 tree shrews were randomly divided into 4 groups: control group, low-dose group (25 mg/kg), middle-dose group (50 mg/kg) and high-dose group(75 mg/kg). Eight tree shrews in each group. The drug groups were intraperitoneal injected with carrageen glue for 3 days. The representation was observed by perusal and signs of collection and analysis system, hemodynamic indexes were detected by hemorrheology fast measuring instrument. The results of activated thromboplatintime(APTT), prothrombin time(PT), thrombin time(TT) and Fibrinogen(FIB) were measured using automated coagulation analyzer. Results: The tree shrews in drug groups were in low spirits. Tongue vein were of enlargement of growth. The Regular probability pain increased and the colors of the tongue, claw and naso-labial area became darker with the dose increasing. Hemorheology results showed that in high-dose group, the whole blood viscosity, packed cell volume, red cell assembling index, casson yield stress and plasma viscosity increased (P<005 or P<001), erythrocyte electrophoresis time prolonged (P<001), but the RBC deformability index decreased (P<001). Compared to the other two groups, the APTT, PT and TT in high-dose group remarkably decreased (P<005 or P<001), but FIB increased (P<001). Conclusion: The model of blood stasis syndrome was successfully built in tree shrews with high-dose carrageen glue.