To observe the effect of high colon dialysis combined with Chinese medicine retentive enema treatment for chronic renal failure (CRF). Methods: Thirty-two patients received conventional treatment (Group A) and 31 patients were given conventional treatment combined with Chinese medicine retentive enema (Group B) and 34 cases were treated by conventional treatment plus high colon dialysis combined with traditional Chinese medicine retentive enema (Group C). The change of urea nitrogen (Bun), serum creatinine (Scr), serum uric acid (UA), endogenous creatinine clearance urinary inhibition (Ccr), Cystatin C (CysC) and parathyroid hormone (PTH) were observed before and after in each group. Results: Group C showed significant difference after treatment in reducing Bun, Scr, UA, CysC, PTH and rising Ccr (P<001). Group B also showed the difference before and after the treatment (P<005), while the effect of Group C was better than that of the Group B (P<005). The improvement in Group A was not good after treatment (P>005). Conclusion: High colon dialysis combined with Chinese medicine retain enema may significantly improve renal function, reduce the PTH, delay the progress of CRF, and is superior to the combination of traditional Chinese medicine enema and conventional treatment.