To explore the pathology of “interconnection of zang-fu viscera” theory with the guide of it, building pulmonary disease animal models and intestinal disease ones and observing the expression and correlation of SP, VIP, iNOS and CGRP in 12 different types of tissue at 12 various chosen time (8 d, 16 d).Methods:Two suites of healthy female SD rats were adopted, 35 rates per suite, and the body weight of each rat is between 110 to 120 g. Every suite was randomized into 5 groups: normal group, food restriction group, water limiting group, high oxygen group and low oxygen group, and the content of CGRP, iNOS, SP and VIP in the lung, spleen, liver, bladder, kidney, serum, stomach, heart, jejunum, ileum, colon, rectum were tested.Results:The tissues related to CGRP were: the lung and the heart(8 d),the lung and the jejunum(16 d), the kidney and the the bladder (8 d), the spleen and the liver (16 d), the heart and the ileum (16 d); The tissues related to iNOS were: the lung and the bladder (16 d), the heart and the rectum (16 d); The tissues related to SP were: the lung and the liver (8 d and 16 d), the lung, the colon and the ileum(8 d), the spleen and the stomach (8 d), the heart and the ileum (8 d); The tissues related to VIP were: the lung and the liver (8 d), the heart and the colon (8 d and 16 d).Conclusion:By illustrating the relationship between the lung and the large intestine, liver, bladder and heart, the theory “the lung and the large intestine being interior-exteriorly related” were enriched, which had great guiding significance to clinical treatment. In addition, CGRP, SP, iNOS, VIP and other indicators showed a close relationship between the heart and the intestine.