Combined with the ITS2 and psbA-trnH and matK sequence to identify the medicinal plant-calanthe，and establish genus sequences which can accurately identify the calanthe.Methods:Extracting total DNA from 6 species of calanthe，which had been collected by the kit method，Then the sequences were amplified and sequenced，Using codencodeV42 software to of splic and shear the sequences，Comparing of all sequences of 6 species of the genus using MEGA50 software,The variable sites,the Kimura 2-Paramter(K2P)genetic distances and the Neighbor-joining(NJ)phylogenetic tree were computed by MEGA50.Results:56 sequences(including 17 GenBank sequences)were obtained f.By analysis，ITS2 sequence and matK sequence of the intraspecific genetic distance is less than the inter species genetic distance，the intraspecific genetic distance and inter species genetic distance of psbA-trnH sequence was coincident.Conclusion:ITS2 sequence and matK sequence can be used as the identification sequence of the partial plants of the calanthe,however psbA-trnH sequence could not be used for the identification of the calanthe.