To determinate the effective ingredient and fingerprint of Dahurian Angelica Root.Methods:A total of 7 batches of Dahurian Angelica Root purchased in May 2012 in our hospital were divided into 4 major groups based on its batches. The effective ingredients were determined by high performance liquid chromatography and established HPLC fingerprint according to the principle of characteristic peaks of Dahurian Angelica Root. The 7 batches were determined by fingerprint and four peaks were determined and compared to evaluate Dahurian Angelica Root’s quality.Results:Hang Dahurian Angelica Root (Chongqing Wanzhou) and Dian Dahurian Angelica Root (Yunnan) were fake the other batches were average or had good quality. Two batches of Yu Dahurian Angelica Root had better quality than the others.Conclusion:The result of the fingerprint detection can make accurate measurement of the effective components of medicinal materials, with high stability, good reproducibility, which is a reliable method on the quality of identification of Dahurian Angelica Root from different habitats. Its rapid and accurate evaluation of the quality of Chinese medicine is recommended.