To explore the mechanism of herbs-partition moxibustion with the matching mu points in treating UC rats based on metabonomic with UPLC-Q/TOF MS. Methods:Sprague-Dawley rats of 27 cases were randomly divided into normal group, model group, herbs-partition moxibustion with the mactching mu points group. The UC rat model was established by drinking DSS aqueous solution. In herbs-partition moxibustion with the matching mu points group, each point moxibustion with 2 moxa cones, once per day, and lasting for 14 days. After moxibustion treatment, all groups urine was analyzed with UPLC-Q/TOF MS. The acquired data were analyzed using principal component analysis (PCA) and partial leastsquares discriminant analysis (PLS-DA).The potential biomarkers and metabolic pathways were analyzed by Metlin(http://metlin.scripps.edu/), KEGG(http://www.kegg.jp), HMDB (http://www.hmdb.ca), MetaboAnalyst 3.0(http://www.metaboanalyst.ca). Results:Under both positive ion model and negative ion model, the separation trends of 3 groups urine were good. Herbs-partition moxibustion with the matching mu points effectively reduced in 6 different metabolites expression, such as L-cysteine, atraric acid、tocopheronic acid, dibutyl malate、L-tyrosine、L-pipecolic acid, which increased in model group. All 6 different metabolites above were mainly related to amino acid metabolic pathways, such as phenylalanine, tyrosine and tryptophan biosynthesis, cysteine and methionine metabolism, tyrosine metabolism, and so on. Conclusion:The mechanism of herbs-partition moxibustion with the matching mu points in treating UC may mainly relate to amino acid metabolism.