To observe the clinical effect of Guhong Injection on cerebral ischemia reperfusion injury and its effect on the tumor necrosis factor (TNF-α). Methods:A total of 60 cerebral ischemia reperfusion injury cases during February 2011 to June 2016 in the Department of Neurology in our hospital were randomly selected by the visiting sequence numbers, and single blind randomized digital table method was used to divide them into observation group and control group, each group with 30 cases. The observation group had 19 female and 11 male patients, aged 41-74, average age (56.04±9.41). Control group, 17 cases female, 13 male cases; age 42-73, average age (53.82±8.26). There were no significant differences in gender and age between the two groups (P>0.05). Motor function was evaluated before and after treatment with Fugl-Mayer assessment table in patients with hemiplegia limb motor function assessment (FMA). Activities of daily living (ADL) of patients was evaluated before and after treatment with Modified Barthel Index (MBI) assessment, including independent living skills proficiency. Tumor necrosis factor alpha (TNF-α) was detected by enzyme linked immunosorbent assay (ELISA). Results:After treatment, the FMA scores of the two groups were higher than before the treatment (P<0.05); the FMA scores of the observation group were significantly higher (P<0.05) than the control group. After treatment, observation group’s MBI questionnaire score improved significantly compared with before treatment and the control treatment group (P<0.01) after treatment, two groups’TNF-α expression levels decreased significantly. The observation group had a significant decrease compared with both observation group’s results before the treatment, and control group after treatment, (P<0.01). As for the correlation analysis between FMA, mBI and TNF-αin patients with cerebral ischemia reperfusion, the results showed that compared with r=-0.971, FMA, P=0.000<0.05, and mBI compared with r=-0.969, P=0.000<0.05, both were negatively correlated. Conclusion:Guhong injection can effectively improve the limb motor dysfunction in patients with cerebral ischemia reperfusion injury, and help decrease the expression level of TNF-α. Its therapeutic mechanism may be related to the down-regulation of TNF-α expression level.