To investigate the effect of Shen Fu injection combined with chemotherapy on hematopoietic and immune function in patients with middle and advanced non-small cell lung cancer (NSCLC). Methods:Totally 92 patients with middle and advanced NSCLC were randomly divided into the control group and the observation group, 46 cases in each. The control group was treated with cisplatin combined with paclitaxel (TP) chemotherapy, and the observation group was with the combination of intravenous infusion of 60 mL/d. Both of the 2 groups were carried out for 4 cycles of chemotherapy. The changes of hematopoietic function, humoral immunity and cellular immune function in 2 groups were detected and compared after chemotherapy. Results:Compared with those before chemotherapy, levels of peripheral white blood cell (WBC), hemoglobin (Hb) and platelet (PLT) of both groups increased firstly and then decreased after 1 week of chemotherapy till the end. And for the observation group, the level of WBC in peripheral blood was significantly higher than that of the control group (P<0.05 or P<0.01), and Hb and PLT level compared with the level of control group had no significant difference, but there was still significant difference with those before chemotherapy (P<0.05); in control group, the levels of serum IgG, IgA and IgM after chemotherapy and CD3+, CD4+T cell ratio and CD4+/CD8+ all decreased, and the percentage of T cells in CD8+ increased (P<0.05 or P<0.01); while in the observation group, levels of serum IgG, IgA and IgM, CD3+, CD4+T cell ratio and CD4+/CD8+ ratio increased significantly, and the proportion of CD8+ T cells decreased significantly (P<0.05 or P<0.01); in addition, the observation group had less rate of leukopenia, nausea and vomiting and other adverse reaction than the control group (P<0.01). Conclusion:Shen Fu injection can effectively reduce the bone marrow suppression and immune suppression in NSCLC patients with chemotherapy drugs, restore the body’s hematopoietic function, and effectively improve the body’s humoral and cellular immune function.