To investigate the effect of Jiegu Pill on the expression of serum calcium, ALP, fracture CD3, CD44 in SD rats with fracture. Methods: Forty SD rats were randomly divided into four groups (control group, model group, low dose group, high dose group) with 10 rats in each group. Model group, low dose group and high dose group were given. Rats in the control group and the model group were given equal volume of ordinary saline and the low dose group was intragastricly administrated with Jiegu Pill solution of 0.4 g/kg concentration, while rats in the high dose group was intragastricly administrated with Jiegu Pill of 1.6 g/kg solution. Results: The serum calcium, ALP, CD3, CD44 levels of the model group, low dose group, high dose group a week and 2 weeks later after the treatment were lower than those in the control group. serum calcium, ALP, CD3, CD44 levels of the model group and the low dose group 3 weeks after the treatment were lower than those in the control group and the high dose group, but the levels of serum were higher in the high dose group compared to those of the control group 3 weeks after the treatment(P<0.05). The levels of serum calcium, ALP, CD3 and CD44 were higher in the high-dose group and low-dose group than those in the control group and the model group after 4 weeks treatment and the high-dose group was superior to the low-dose group (P<0.05). Conclusion: Jiegu Pill may increase the expression of serum calcium, ALP, CD3, CD44 and promote fracture healing.