To evaluate the genotoxicity of Jianpi Shengxue Granule.Methods:Genotoxicity of Jianpi Shengxue Granule was studied using Ames test,CHO-K1 cell chromosomal aberration test,and mouse bone marrow cell micronucleus assay,respectively.Results:The Ames test results indicated that Jianpi Shengxue Granule did not affect the normal growth of tested bacteriums at all dosages with or without S9.Compared with the negative control group,all number of reversion mutation had no significant up-regulation,and no dose-response relationship was found.Results of CHO-K1 cell chromosomal aberration test indicated that the aberration rates of Jianpi Shengxue Granule group(650,130,and 26 μg/mL)were all less than 5%.Results of micronucleus assay indicated that there was no statistical difference of micronucleus rate between the Jianpi Shengxue Granule group(2 000,1 000,and 500 μg/mL)and negative group(P>0.05).Conclusion:No genotoxicity of Jianpi Shengxue Granule was found under the conditions of this study.