To study the method and condition for HPLC fingerprinting of Radix Puerariae, in order to better control the quality of herbal medicine. Methods:Agilent 1260 by high performance liquid chromatograph was applied and kromasil 100-5C18 (4.6 mmx250 mm, E63848, 25 cm) chromatographic column was established. The mobile phase was A-acetonitrile and B-0.1% phosphoric acid aqueous solution with gradient elution and velocity was set as 1 mL/min with the column temperature for 30 ℃. The detection wavelength was 250 nm to explore and study Radix Puerariae extracts of medicinal herbs as well as fingerprints. Results:Separation in each chromatographic peak of obtained chromatogram by the above method was great, meeting the requirements to fingerprints. The fingerprint for Radix Puerariae was preliminary established. Conclusion:As different HPLC fingerprint methods are applied, the map results will vary. The optimal use and ideal conditions of high performance liquid chromatography method can be summarized based on the result of the best fingerprints.