To observe the clinical effects of Jianpi Tiaogan Wenshen Formula on IBS-D and influence on blood biochemical index.Methods:A total of 122 cases of IBS-D were randomly divided into traditional Chinese medicine group (n=61) and Western medicine group (n=61).The Chinese medicine group was treated with Jianpi Tiaogan Wenshen Formula，the western medicine group were given pinaverium bromide tablets.Two groups were treated for four weeks.VAS scale was used to evaluate the degree of abdominal pain before and after treatment，the serum levels of inflammatory cytokines，immune function，gastrointestinal hormones，brain-gut peptide and intestinal mucosal MC were measured before and after treatment.Results:After treatment，the levels of IFN-γ，IL-8，IL-12，CD8+ T cells，MOT，GAS，5-HT，SP，VAS score，stool character score and MC numbers significantly decreased，IL-10，CD3+，CD4+，CD4+/CD8+ T cell ratio，VIP，CCK and CGRP levels significantly increased (P<0.05 or P<0.01)，and there were significant differences between the two groups (P<0.05 or P<0.01).Conclusion:Jianpi Tiaogan Wenshen Formula can significantly improve the gastrointestinal peristaltic function of IBS-D patients，balance the levels of inflammatory cytokines in body，reduce the number of MC in intestinal mucosa and the release of 5-HT，then alleviate the clinical symptoms，the curative effect is remarkable，which is worthy of clinical application.