To observe the clinical effect of acupuncture combined with ear-acupuncture on patietns with female chloasma. Methods:Sixty-eight female with chloasma in our hospital (from Janury 2014 to June 2015) were randomly selected and divided into two groups. Thirty-four patients treated with vitamin C and vitamin E were taken as control group, and the other 34 patients treated with acupuncture and ear acupoint were taken as observation group. Compared the clinical effects and the skin lesions integration before and after treatments within two group. In addition, detect the levels of serum E2 and LH a week before menstruation before and after treatments. Results:The overall efficiency were 88.23% in observation group, which was higher than that of the control group, and differences is statistically significant (P<0.05). The skin lesions integration was (2.46±0.87) in observation group after treatments while that in control group was (3.21±0.94), and the difference is statistically significant (P<0.05). The level of serum E2 was (28.46±12.08)pg/mL in observation group after treatments compared to (54.23±13.25)pg/mL of the control group, and the difference is statistically significant (P<0.05). The level of serum LH was (5.26±1.58) miu/mL in observation group after treatments while that of the control group was (7.82±1.69)miu/mL with statistical significance (P<0.05). Conclusion:Acupuncture and ear-acupoint can improve the levels of serum E2 and LH with exact effect in the treatment of chloasma, which is worthy of clinical application.