To observe the mechanism of garcinia yunnanensis extract on subcutaneously implanted tumor of human colorectal carcinoma in nude mouse.Methods:A nude mouse model of subcutaneously implanted tumor of human colorectal carcinoma was established,and experimental nude mice were randomly divided into 5 groups：model group,positive control group (5-Fluorouracil),and high/middle/low doses of garcinia yunnanensis group.After 28 days of treatment,the tumor was cut to weigh and anti-tumor rate was calculated,and the apoptosis ratio of tumor cells was analyzed.CyclinE1,c-Met,hnRNPK expression level were analyzed by immuno-histochemical method.Results:The experimental results showed that,the anti-tumor rates in low/middle/high doses of garcinia yunnanensis group were 12.5%,20.83% and 29.17%,which were significantly different from the normal control group (P<0.01).The apoptosis rate of tumor cells was increased with the increasing of extract concentration of garcinia yunnanensis,which showed a dose-dependent manner (P<0.05).Garcinia yunnanensis could significantly reduce the expression of cyclinE1,c-Met and hnRNPK with a dose-dependent manner (P<0.01).Conclusion:Garcinia yunnanensis has an obvious anti-tumor effect in vivo,and it may inhibit the proliferation of tumor cells in vivo by reducing the expression of cyclinE1,c-Met and hnRNPK.