To observe the effects of electroacupuncture(EA) on depressive-like behavior and its mechanism.Methods:Eighteen male WKY rats were randomly divided into EA,Sham,and model groups.Six Wistar rats were divided into normal group.The behaviors were evaluated by sucrose preference test and force swimming test after 21 days,and the expression of β-CaMKⅡ was detected by immunofluorescence.Results:Compared with the control group,the level of β-CaMKⅡ expression of hippocampus in the model group decreased significantly along with Sucrose preference test(P<0.05).Meanwhile,immobile time of force swimming test increased significantly (P<0.05).Compared with sham group,EA group increased significantly in the β-CaMKⅡ expression of hippocampus and sucrose preference test (P<0.05)，and decreased significantly in immobile time of force swimming test(P<0.05).Conclusion:Electroacupuncture can improve the WKY rats′ depressive-like behaviors and the expression of β-CaMKⅡ protein in hippocampus.This might be one of the mechanisms of Electroacupuncture on depression which is mediated by hippocampus.