To observe the changes of CD series in different age groups after herbal moxibustion on CV 8 (Shenque) and CV 4 (Guanyuan) and so on, and to explore the mechanism of moxibustion regulating body immunity. Methods:Liuwei Dihuang Pill herbal powder were made into herbal mox cake and CV 8 (Shenque), CV 4 (Guanyuan), ST 36 (Zusanli), BL 20 (Pishu) and Bl 23 (Shenshu) were selected. The moxa cone (0.3 g) was placed right on the herbal cake over the selected acupoints and burned. In each treatment, three cones were applied to each acupoint. The treatment was carried out once every other day for 10 times. Each subject was extracted venous blood about 5 ml before and after all the treatment. Results:After the treatment with herbal cake moxibustion, CD3, CD4, and CD4/CD8 were increased (P>0.05) in young people; in the middle-aged group, CD3 was increased (P>0.05), CD4 and CD4/CD8 were increased, however, CD8 was decreased (P<0.05); in the elder group, CD3 and CD4/CD8 were increased (P<0.05), CD8 was decreased (P<0.05), and CD4 had little increase (P>0.05). Conclusions:The regulation of herbal cake moxibustion on body immunity in different age groups was significant in the elder group and middle-aged group, and the mechanism was different in each group.