To establish the determination method of baicalin and chlorogenic acid dissolution in Yinhuang tablets so as to provide criteria and parameters for the quality control of Yinhuang tablets. Methods:The dissolution of chlorogenic acid and baicalin from Yinhuang tablets was determined by HPLC method with DAD detector and Agilent TC-C18 column (250 mm ×4.6 mm, 5 μm) by using mixture of acetonitrile and purified water both containing 0.05% TFA as mobile phase with gradient elution in the current speed of 1.0 mL/min, and the detection wavelength of baicalin and chlorogenic acid were 276 nm and 327 nm, respectively. The dissolution test was operated according to the second dissolution method-oar method recorded on the Chinese medicine pharmacopoeia 2015 edition. Results:It was demonstrated that there was a good linear relationship in the range of 0.1-20 μM in baicalin and 0.5-30 μM in chlorogenic acid (R2=0.9996), respectively. The dissolution of chlorogenic acid and baicalin from Yinhuang tablets could be determined at the same time by the same HPLC method. Conclusion:The HPLC method was rapid, sensitive, accurate, and reproducible. It could be used to determine the baicalin and chlorogenic acid dissolution in Yinhuang tablets and provide important references for the quality control of Yinhuang tablets.