To investigate the relationship between TCM syndromes and liver function, serum virology, immunohistochemical HBsAg, HBcAg detection rate and liver histopathology in patients with chronic hepatitis B (CHB) and its diagnostic significance for CHB. Methods:Fifty-eight cases of CHB patients who were hospitalized in our department from June 2013 to August 2016 were selected, comparing the relationship between different TCM syndromes and liver function, HBVDNA level, immunohistochemical HBsAg, HBcAg detection rate and liver histopathology. Results:1)TCM syndrome differentiation：liver depression and spleen deficiency syndrome (26 / 58,31%) and damp-heat syndrome (15 / 58,30%) appeared with the highest frequency, followed by liver and kidney yin deficiency syndrome (7/58, 15%), spleen and kidney yang deficiency (6 / 58,12%) and blood stasis in collaterals syndrome (4 / 58,12%); 2)different TCM syndromes and ALT were significantly correlated, with significant difference in comparison (P<0.05); there was no significant correlation between different TCM syndromes and AST and TBIL, and no statistical difference (P>0.05); 3)different TCM syndromes and HBV DNA were significantly correlated, with significant difference in comparison (P<0.05); there was no significant correlation between HBsAg and HBeAg in different TCM syndromes, with no statistical difference (P<0.05); 4)there was correlation between different TCM syndromes and immunohistochemical HBcAg detection rate (P<0.05) with various groups shared significant difference, while not between different TCM syndromes and immunohistochemical HBsAg detection rate (P>0.05); 5)liver inflammation G and fibrosis degree S of CHB patients were highly correlated,( r=0.758, P<0.01); different TCM syndromes and liver inflammation G were significantly correlated (P<0.05); different TCM syndromes and fibrosis Degree S was significantly correlated ( P<0.01). Conclusion:The detection rate of ALT, HBV DNA, immunohistochemical HBcAg and liver inflammation G and fibrosis degree S in CHB patients are significantly correlated with TCM syndromes, which is of great significance to guide the clinical diagnosis and treatment of CHB patients.