Professor Wang Juyi proposed that meridians are “Muscle divisions” to indicate the crevices between all of the different viscera and tissues in the body and qi transformation is the most important function of meridian,to expound three Yin’s and three Yang’s meridian function of opening-closing-regulating. He put forward “symptoms-structure” theory to set forth that the meaning of “meridian-changes of diseases” and “meridian-indications” to explain the relationship between “symptoms-structure” and “meridian-changes of diseases” “meridian-indication”. Prof. Wang developed the methods of meridian diagnosis to elect the meridian and acupoints to treat diseases,etc. This paper is to analysis Professor Wang Juyi’s academic thoughts and acupuncture and moxibustion in meridian and collateral differentiation diagnosis-treatment system.