To observe the protective effect of Danshen A sodium sulfonate injection on myocardial ischemia and reperfusion injury in rabbit,investigate the effect of Danshen A sodium sulfonate injection on myocardial enzyme spectrum and explore its potential mechanism to heart function.Methods:Twenty-four rabbits were randomly divided into ischemia/reperfusion group,ischemic preconditioning processing group A,and ischemic preconditioning group B.Ischemic preconditioning processing group A was injected via ear vein with Danshen A sodium sulfonate injection at 2.5 mL/kg/d,while ischemic preconditioning treatment group B at 5 mL/kg/d and ischemia/reperfusion group were injected with 1 mL physiological saline.After a week preprocessing,rabbits in all groups were ligated at coronary artery and was re-perfused for 30 minutes,30 minutes after ligation.The rest 8 rabbits were fed up as control group.Vascular endothelial growth factor (VEGF) and VEGF mRN expression before and after processing were measured by double antibody sandwich EUS,and serum total superoxide dismutase (T-SOD),lactate dehydrogenase (LDH),creatine kinase isoenzyme (CK-MB) and myocardial malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELIS).The protective effect of Danshen A sodium sulfonate injection on vascular endothelial injury and its effect on myocardial enzyme spectrum in rabbits after myocardial ischemia preconditioning were evaluated.Results:Differences of the four groups in ischemic preconditioning showed no significance (P>0.05),VEGF and mRN expression in both group A and B after preprocessing were significantly enhanced,and T-SOD,LDH and CK-MB of were obviously improved and the release of MD declined in the two groups with statistically significant difference compared with the ischemia/reperfusion group (P<0.05).There was no significant difference between the ischemic preconditioning group A and group B (P>0.05).Conclusion:Danshen A sodium sulfonate injection on myocardial ischemia and reperfusion injury in rabbits may significantly increase VEGF and enhance LDH activity,accelerate myocardial ischemia vascular endothelial growth,inhibit lipid peroxidation inhibition and reduce myocardial vascular endothelial inflammatory response,thereby improving impaired endothelial function due to ischemia/reperfusion injury.It has clear protective effects on rabbit experimental myocardial ischemia/reperfusion injury.