To guide the clinical application of antimicrobial drugs and the administration basis for appropriate measures of nosocomial infection by monitoring the antimicrobial resistance of clinical bacterial isolates in 3 first class hospitals of traditional Chinese medicine in Beijing for three years. Methods:Pathogenic bacteria of nosocomial infection were collected from January 1 st， 2014 to December 31 st， 2016 in Beijing Hospital of Traditional Chinese Medicine， Shunyi Branch of Beijing Hospital of Traditional Chinese Medicine and Pinggu Branch of Beijing Hospital of Traditional Chinese Medicine. Regular methods were used to isolate and cultivate the pathogenic bacteria of nosocomial infection， and the types of strains were identified while antimicrobial resistance of bacteria was measured by antibiotic susceptibility test. CLSI-2010 document was the standard reference and the WHONET 5.4 software was used for data analysis. Results:A total of 21，114 strains were isolated by the 3 hospitals according to the surveillance project. A total of 13，971 strains， 66.2% of the amount， were of Gram-negative， including 2，787 strains of Escherichia coli accounting for 19.9% and then 2，350 strains of Neisseria accounting for 16.8%. And 7，143 strains， 33.8% of the amount， were of Gram-positive， including 3，013 strains of α-hemolytic streptococcus accounting for 42.2% and 1，542 strains of Staphylococcus aureus accounting for 21.6%. Conclusion:Based on the basic data platform for the monitoring of antimicrobial resistance of nosocomial bacteria， the status of bacteria resistance in the 3 monitoring hospitals are now generally detected. It’s common that the clinical bacterial isolates have resistance to drugs， and the resistance rate is increasing but stable in the past three years. Escherichia coli， Staphylococcus aureus， Pseudomonas aeruginosa， etc. should remain focused on monitoring， and the control measures of nosocomial infection should be strengthened to curb the spread of drug-resistant strains.