Experimental autoimmune encephalomyelitis (EAE) model is an internationally recognized classic animal model of multiple sclerosis (MS) and is widely used in researches on the pathogenesis of MS and the evaluation of immunomodulator for MS. However， There are still many defects leading to morbidity less than 100% and high mortality. Therefore， in order to reach a high morbidity， a low mortality and a stable EAE model， the process of EAE model establishment was standardized and refined by improving the preparation， operation and nursing of the model immunization. In addition， the key issues to the successful establishment were analyzed and the solutions were provided according to the process of experiment operation.