To observe the effect of “Danzhi Tongzhu” in clinical treatment of depression after stroke. Methods:A total of 60 patients with post-stroke depression admitted in departments of acupuncture， rehabilitation and stroke disease of Xinle Hospital of Traditional Chinese Medicine from March 2015 to February 2016 were randomly divided into a treatment group and a control group according to the order of visits， with 30 patients in each group. Patients in the control group were given oral administration of fluoxetine hydrochloride (20 mg， 1 times a day) on the basis of routine medical treatment， while patients in the treatment group were given Danzhi Tongzhu on the same basis. Before treatment， 30 days and 60 days after treatment， Fugl-Mayer assessment scale (FMA) was used to assess the motor function of hemiplegic limbs of patients; the Hamilton depression scale (HAMD) was used to evaluate the depression of patients; activities of daily living scale (mBI) was used to assess the quality of life of patients and enzyme-linked immunosorbent assay (ELISA) was used to detect the patients’ brain-derived neurotrophic factor (BDNF). Results:After treatment， the FMA， mBI score and BDNF were all higher than those before treatment (P<0.05). Compared with the control group， there was no statistically significant difference in the FMA， mBI score and BDNF in the treatment group (P>0.05) 30 days after treatment， while 60 days after treatment， the FMA， mBI score and BDNF were higher (P<0.05). By contrast， the HAMD score was negatively correlated with the BDNF expression and the HAMD scale score， and the correlation coefficient of Pearson was-0.949， P=0.000<0.05. Conclusion:Danzhi Tongzhu can significantly improve PSD symptoms of depression， and therefore further assist patients with recovery of body function， and the effect may be related to the increase in BDNF.