To observe the effect of serum containing Erxian decoction on regulating the expression of bcl-2and bax protein from rats’ ovarian granulosa cells induced by cisplatin and to investigate the mechanism of Erxian Decoction serum on apoptosis of ovarian granulosa cells from the perspective of apoptosise. Methods:Thirty SD female rats for 22 to 24 days birth were randomly divided into three groups：the normal saline group，progynova group and the Erxian Decoction group with 10 rats in each group. After 4-day drug administration，rat hearts were taken to prepare drug serum. Ovarian granulosa cells were taken from SD rats and cultured to acquire adherent cells. Then，primary ovarian granulosa cells in rats were induced by cisplatin. After pharmacological action to serum，radio immunity method was used to detect estrogen and progestin and make analysis. Immunohistochemistry was applied to detect the expression of bcl-2 and bax protein in each group and then for image analysis. Results:Test results showed that E2 expression significantly changed in the model group and progynova group (P<0.05) with statistically significant difference，compared with the normal group. Compared with the model group，the expression of E2 increased significantly in the progynova group and the Erxian Decoction group. Compared with the normal group，P expression in the model group was somewhat changed (P<0.05)，which showing statistically significant difference. Compared with the model group，P expression in Erxian Decoction group was slightly higher than that of the progynova group，but lower than that of the normal group. Immunohistochemistry results showed that brown granules of bcl-2 and bax protein were expressed in the cytoplasm of ovarian granulosa cells. Compared with the normal group，the expression of bcl-2 in each group under the intervention of cisplatin was decreased，and the model group was the most obvious (P<0.05).Compared with model group，the positive expression of bcl-2 in each drug group was increased，but it was weaker than that in the normal group (P<0.05). The intensity of bax and bcl-2’s expression was opposite. Compared with the normal group，Bax expression in each group under the intervention of cisplatin were enhanced and that in the model group especially showed a strong expression (P<0.05). Compared with the model group，the positive expression of the drug groups was decreased，but was higher than that of the normal group (P<0.05) with statistically significant difference. Conclusion:Erxian Decoction can decrease the expression of Bax protein in follicular granulosa cells induced by cisplatin injury and increase the expression of bcl-2 protein. It can inhibit the apoptosis of ovarian granulosa cells and protect the ovarian function by adjusting the expression of bcl-2 and bax.