To study the effect of Wang’s Baochi Pill combined with probiotics and zinc preparation on serum and colonic lavage indicators in treating pediatric persistent diarrhea. Methods: A total number of 106 children with persistent diarrhea in the hospital from March 2013 to April 2016 were randomly divided into control group and treatment group, with 53 cases in each group. The control group received routine treatment and probiotics and zinc preparation, while the treatment group additionally received Wang’s Baochi Pill. The levels of serum and colonic lavage indicators were compared between the two groups before and after treatment, and the clinical efficacy was analyzed. Results: The levels of IL-6, IL-8, NO and MDA in serum and colonic lavage fluid after treatment were lower than those before the treatment （P<0.05）. The levels of superoxide dismutase （SOD） and serum zinc were higher than those before the treatment and the difference in the two groups was significant （P<0.05）, and the treatment group was closer to the normal compared with the control group. The total effective rate of treatment group was 86.79% which was higher than that of control group （62.26%）, showing statistically significant difference between the two groups （P<0.01）. Conclusion: Wang’s Baochi Pill combined with probiotics and zinc preparation in the treatment of persistent diarrhea for children have better effect in serum and colonic lavage related indicators, which is worth of promotion.