To observe the efficacy of ultrasound drug penetration in the treatment of NAFLD. Methods:A total of 30 patients were randomly divided into two groups. The treatment group were treated by ultrasound drug penetration of spleen-tonifying and liver-soothing medicine and the control group by ultrasound ion penetration. The treatment lasted for three months. Then, three-months’ follow-ups were performed for each patient. The monitored indexes included BMI, serum transaminase, liver/spleen CT ratio and clinical symptoms. Results:Both treatments in the two groups effectively improved the serum ALT, AST levels, reduced the patient’s liver/spleen CT value, improved the patient’s BMI, and relieved the clinical symptoms including liver pain, abdominal distention, and fatigue （P<0.001）. Conclusion:The formula in the treatment of NAFLD has a good effect with the long-term efficacy reflected in diet and sports.