To compare the fingerprints among Medicinal Cyathula Root raw products, distilled by solid water and different concentrations of ethanol, so as to stud the effects of Medicinal Cyathula Root products processed by different concentration of ethanol. Methods:By using HPLC method, taking the SHIMADZU VP-ODS （4.6×250 mm, μm） as the chromatographic column, acetonitrile-water as mobile phase gradient elution, flow rate 0.8 mL/min, column temperature 25 ℃,detection wavelength 243 nm, sample volume 15 μL, acquisition for 90 min. Results:Most of the peak area of Medicinal Cyathula Root that processed by ethanol were slightly higher than raw products; raw products were lower than the distilled water products, but not very obviously. Conclusion:In this experiment, Medicinal Cyathula Root processed by ethanol is conducive to the dissolution of most active ingredient, high concentration ethanol processed product is better than the low concentration ethanol in the terms of the active ingredients dissolution.