To observe the expression of c-fos mRNA of rats with cerebral infarction in recovery period under supplementing qi and activating blood circulation drugs. Methods:Longa method was slightly modified in making the rat model of left middle cerebral artery occlusion. According to the random number table method，the rats were randomly divided into 7 groups，including a normal group, a model group, a sham operation group, a supplementing qi group, an activating blood circulation group, a supplementing qi and activating blood circulation group, and an ERK inhibitor group. Using RT-PCR technique, we observed the effect of supplementing qi and activating blood circulation drugs on c-fos mRNA of rats with cerebral infarction after 28 days' treatment in myocardial infarction cortex. Results:After 28 days' treatment，compared with normal group, c-fos mRNA expression in the model group was significantly decreased (P<0.05)；c-fos mRNA expression in the supplementing qi and activating blood circulation group and ERK inhibition group increased to a certain extent than that in the model group (P<0.05)；the activating blood circulation group had no significant statistical difference with the model group(P>0.05)；c-fos mRNA expression in supplementing Qi group was lower than the other groups (P<0.05). Conclusion:In the recovery period of cerebral infarction, supplementing qi and activating blood circulation drugs can improve cerebral ischemia in a certain degree, which is better than simply supplementing qi and activating blood circulation, and its therapeutic mechanism may be related to improving the expression of c-fos mRNA.