To observe the clinical curative effects of “Intelligence Seven Needles” on vascular cognitive impairment of non-dementia (VCIND),and to explore the partial mechanism.Methods:A total of 60 cases of patients with VCIND were included and randomly divided into the control group and the treatment group, 30 cases in each group.Both of the two groups were receiving oral administration of donepezil (5 mg/time, qd) or huperzine A (100 μg/time, bid), and the treatment group was added “Intelligence Seven Needles”, once a day, 30 min per time, and5 times per week.After continuous treatment for 3 months, the changes of Montreal scale (MoCA), digit span (DST), auditory verbal learning test (AVLT) and voice fluency test (VFT) between the two groups at the time that before treatment, 1 month and 3 months after treatment were compared, and trademark protein expression changes of JNK signaling pathway in the peripheral blood before and after the treatment between the two groups were detected.Results:1) MoCA score, AVLT score and VFT score were significantly increased after 1 month's treatment, and the differences were statistically significant compared with control group (P<0.05).The DST scores were improved in two groups after treatment (P<0.05), but the scores were similar in the two groups after treatment (P>0.05).MoCA, AVLT and VFT in the treatment group after three months' treatment were better than that in the control group, and the differences were statistically significant (P<0.05).There were no statistical differences of DST scores between the two groups after 3 months' treatment (P>0.05).2) The concentration of pJNK in the two groups decreased after treatment, and the decrease trend of the treatment group was more significantly (P<0.05).Conclusion:“Intelligence Seven Needles” can significantly improve the cognitive ability of patients with VCIND, and its mechanism may be associated with the lower of JNK phosphorylation.