To study the acute toxicity of 95% ethanol extracts from Dryopteris fragrans (L.) Schott in mice. Methods:The Dryopteris fragrans 95% ethanol extracts were given to the healthy Kunming mice by intragastric administration. The dose range of 95% ethanol extracts were 10.00 g/kg~24.00 g/kg. The movement and toxic symptoms were observed and the number of dead mice were recorded. LD50 were calculated by Bliss' method. Pathological changes were examined by pathological section. Results:LD50 of the 95% ethanol extracts were 13.53 g/kg, respectively. Pathological section indicated that the myocardial coagulation necrosis, renal tubular degeneration and necrosis, and mild swelling of liver cells, vacuole degeneration, while other organs such as the spleen and lung were with no significant pathological changes. Conclusion:The 95% ethanol extracts of Dryopteris fragrans possesses certain toxicity.