To investigate the tumor inhibited effect and acute toxicity to mice by Huoxue Yiqi Fang Kangliu Wan (KLW) prepared by hospital.Methods:A plenty of glioma C6 cells were injected into right side of nude mice to establish nude mice model of bearing cancer.According to tumor volume，the nude mice were randomly divided into two groups，and separately to irrigate mice with high or low dose KLW.The temozolomide was as positive group and solvents (pure water) was as negative group.The weight and tumor volume of mice were investigated and symptoms were observed before and after medication.After continuous administration for 15 days，the mice were killed and tumors were weighted，and Western blot was used to detect Bcl-2，Bax.ICR mice were irrigated with KLW twice a day，then the general conditions，toxic reaction and death condition before administration and after administration 4 h and 14 days was reviewed with naked eyes.At last the mice were anatomized and organ changes of mice were observed.Results:All nude mice were alive in medication session with weight added，and there was no significant abnormality in general behavioral observation.After administration for 15 days，the tumor volume and weight were significantly declined in both high and low dose group.The scale model of Bcl-2/Bax of control group significantly grew.All administration group mice were alive and there was no significant abnormality in general behavioral observation in the period under observation.There was no significant difference between the two sexes of mice and the negative control group (P<0.05).Anatomical observation on each mice's organ found no abnormality.Conclusion:Both high and low dose KLW have significant inhibiting effect on the growth of xenograft of C6 cells，and this effect was related to induced glioma apoptosis.It also has statistical significance compared with negative control group.KLW dose is 40 mL/kg BW and maximum dose is 0.50 g/mL，which are 107 times of human clinical dose.There is no significant toxicity effect，so it is safe.